A culmination of much of Leonardo's notebook entries and thoughts on painting appears in his Treatise on Painting. Included in the book is a section known as Botany for Painters and Elements of Landscape Painting. It is in this section that Leonardo reveals three discoveries about botany. These discoveries include the arrangement of leaves on a stem, how to determine the age of a tree by its structure, and that new branches form from underneath the bark of a tree. His findings became a staple for future botany and also instructed artists on how to properly display plants in paintings.